Journal: Genes

Article Title: A New Function of the DRD1 Gene: GnRH Secretion Regulation in Sheep Hypothalamic Neurons

doi: 10.3390/genes16030273

Figure Lengend Snippet: Primers for detecting mRNA levels via real-time quantitative PCR.

Article Snippet: The recombinant plasmid pEGFP-C2- DRD1 was subjected to endotoxin-free large-scale extraction (Tiangen) and concentration measurements and was packaged at −20 °C for storage to await cell transfection.

Techniques: Sequencing

Journal: Genes

Article Title: A New Function of the DRD1 Gene: GnRH Secretion Regulation in Sheep Hypothalamic Neurons

doi: 10.3390/genes16030273

Figure Lengend Snippet: DRD1 interference fragments.

Article Snippet: The recombinant plasmid pEGFP-C2- DRD1 was subjected to endotoxin-free large-scale extraction (Tiangen) and concentration measurements and was packaged at −20 °C for storage to await cell transfection.

Techniques: Sequencing

Journal: Genes

Article Title: A New Function of the DRD1 Gene: GnRH Secretion Regulation in Sheep Hypothalamic Neurons

doi: 10.3390/genes16030273

Figure Lengend Snippet: Primers for detecting caspase-9 mRNA levels via real-time quantitative PCR.

Article Snippet: The recombinant plasmid pEGFP-C2- DRD1 was subjected to endotoxin-free large-scale extraction (Tiangen) and concentration measurements and was packaged at −20 °C for storage to await cell transfection.

Techniques: Sequencing

Journal: Genes

Article Title: A New Function of the DRD1 Gene: GnRH Secretion Regulation in Sheep Hypothalamic Neurons

doi: 10.3390/genes16030273

Figure Lengend Snippet: mRNA expression of DRD1 gene. ** p < 0.05.

Article Snippet: The recombinant plasmid pEGFP-C2- DRD1 was subjected to endotoxin-free large-scale extraction (Tiangen) and concentration measurements and was packaged at −20 °C for storage to await cell transfection.

Techniques: Expressing

Journal: Genes

Article Title: A New Function of the DRD1 Gene: GnRH Secretion Regulation in Sheep Hypothalamic Neurons

doi: 10.3390/genes16030273

Figure Lengend Snippet: Construction and validation of recombinant plasmids. ( a ) Double enzyme digestion of pEGFP-C2-DRD1. 1: results of double enzyme digestion for pEGFP-C1-DRD1; M: the DNA marker DL10000. ( b ) Inverted microscope image after pEGFP-C2-DRD1 transfection. ( c ) Inverted microscope image after pEGFP-C2-DRD1 transfection.

Article Snippet: The recombinant plasmid pEGFP-C2- DRD1 was subjected to endotoxin-free large-scale extraction (Tiangen) and concentration measurements and was packaged at −20 °C for storage to await cell transfection.

Techniques: Biomarker Discovery, Recombinant, Marker, Inverted Microscopy, Transfection

Journal: Genes

Article Title: A New Function of the DRD1 Gene: GnRH Secretion Regulation in Sheep Hypothalamic Neurons

doi: 10.3390/genes16030273

Figure Lengend Snippet: Analysis of DRD1 mRNA expression in sheep hypothalamic neurons. ( a ) Cell morphology before interference; ( b ) blank control group; ( c ) mRNA expression after DRD1 siRNA interference; ( d ) mRNA expression of the DRD1 gene; ** indicates extremely significant differences.

Article Snippet: The recombinant plasmid pEGFP-C2- DRD1 was subjected to endotoxin-free large-scale extraction (Tiangen) and concentration measurements and was packaged at −20 °C for storage to await cell transfection.

Techniques: Expressing, Control

Journal: Genes

Article Title: A New Function of the DRD1 Gene: GnRH Secretion Regulation in Sheep Hypothalamic Neurons

doi: 10.3390/genes16030273

Figure Lengend Snippet: Immunofluorescence staining for detection of DRD1 expression after transfection: ( a ) blank, ( b ) siDRD1, and ( c ) pEGFP-C2-DRD1.

Article Snippet: The recombinant plasmid pEGFP-C2- DRD1 was subjected to endotoxin-free large-scale extraction (Tiangen) and concentration measurements and was packaged at −20 °C for storage to await cell transfection.

Techniques: Immunofluorescence, Staining, Expressing, Transfection

Journal: Genes

Article Title: A New Function of the DRD1 Gene: GnRH Secretion Regulation in Sheep Hypothalamic Neurons

doi: 10.3390/genes16030273

Figure Lengend Snippet: The relative expression of DRD1 and related genes; ** indicates extremely significant differences, * indicates significant differences.

Article Snippet: The recombinant plasmid pEGFP-C2- DRD1 was subjected to endotoxin-free large-scale extraction (Tiangen) and concentration measurements and was packaged at −20 °C for storage to await cell transfection.

Techniques: Expressing

Journal: Genes

Article Title: A New Function of the DRD1 Gene: GnRH Secretion Regulation in Sheep Hypothalamic Neurons

doi: 10.3390/genes16030273

Figure Lengend Snippet: Simple pathway through which DRD1 is involved in GnRH secretion.

Article Snippet: The recombinant plasmid pEGFP-C2- DRD1 was subjected to endotoxin-free large-scale extraction (Tiangen) and concentration measurements and was packaged at −20 °C for storage to await cell transfection.

Techniques: